Development and Validation of Rp-hplc Method for Thesimultaneous Estimation of Amlodipine Besylate and Clopidogrel in Bulk and Tablet Dosage Forms

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of amlodipine and clopidogrel in table formulation. The separation and quantification was achieved by thermo hypersil BDS C18 250x4.6x5mm column in isocratic mode, with mobile phase consisting of buffer-methanol(30:70v/v). The mobile phase was pumped at a rate of 1.5 ml/min and the detection was carried out at 238nm. The retention time of amlodipine and clopidogrel was found to be 1.9 and 3.8 min, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, LOD, LOQ and robustness. Linearity was observed in the concentration range of 20-60 µg/mL and 300-600 µg/mL. Calibration curve were linear over studies ranges with correlation coefficient found to be 1.00. The developed method was found to ve accurate, precision and selective for simultaneous estimation of amlodipine and clopidogrel in tablets.